Motivation

In order to use full-length sequencing for genotyping of HLA and KIR, it is essential that novel alleles are characterised and submitted in full length and that known alleles are extended to cover the complete length of the gene. However, the manual annotation and submission of fulllength sequences to the European Nucleotide Archive (ENA) and the IPD-IMGT/HLA or IPD-KIR databases is a time-consuming and error-prone task. In 2016 we presented TypeLoader, a tool for automated full-length submission of novel HLA alleles. Here, we present an update, TypeLoader2, with new features and extended capabilities.

Conclusion

TypeLoader2 can easily be used on standard PCs without dependencies on other software.

With the added internal database, TypeLoader2 can be used as a lab’s central information platform on their novel full-length sequences.

We hope that the increased convenience and scope of TypeLoader2 will foster the submission of more full-length sequences to the IPD-IMGT/HLA and IPD-KIR databases, ultimately promoting the widespread use of full-length sequencing for genotyping of both HLA and KIR.

Download from GitHub: https://github.com/DKMS-LSL/typeloader